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    Embryonic development; Live.  
    Watching fruit fly's embryonic development live
Watching fruit fly's embryonic development live

 

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Theodora Koromila
Postdoctoral Fellow, BBE

Using Drosophila embryos, we show how transcriptional repressors contribute to gene expression dynamics. Image is a snapshot from live RNA-imaging of short-gastrulation enhancer driven reporter output at nuclear cycle 13. This imaging technique presents an improved quantitative tool for live-imaging analysis (MS2-MCP system), to allow assay of spatiotemporal expression dynamics. Nuclear membranes were visualized by Nup-RFP-fluorescence (red), while nascent transcripts in the nucleus by GFP-fluorescence (blue dots). Image by Theodora Koromila, PhD.

koromila@caltech.edu

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